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automacs running buffer  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec automacs running buffer
    Automacs Running Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 366 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macs+buffer/autoMACS+Running+Buffer+-+MACS+Separation+Buffer/us12692546-503-17-20
    Average 97 stars, based on 366 article reviews
    automacs running buffer - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Magnetic Cell Separation:

    Article Title: NOD1/2 signaling in macrophages drives adaptive immune resistance in cancer.
    Article Snippet: Single-cell suspensions were generated, and CD45+ immune cells were enriched via magnetic bead-based positive selection using a mouse CD45 TIL MicroBead Kit (130-110-618; Miltenyi Biotec, Bergisch Gladbach, Germany) and a Dead Cell Removal Kit (130-090- 101; Miltenyi Biotec) according to the manufacturer’s instructions. .. Briefly, up to 107 cells were resuspended in 90 μL of MACS Buffer (Miltenyi Biotec) per sample, incubated with 10 μL of microbeads for 15min at 4 °C, and passed through LS columns (130–042–401) mounted on QuadroMACS Separators (Miltenyi Biotec). ..

    Article Title: Dynamics of ML-based Morphological Features Indicate a Shear Stress-Dependent Bifurcation of hiPSC-Derived Endothelial Cell States
    Article Snippet: Alternatively, AICS-0177 CDH5 exon 3 deletion cells and AICS-0126 sorting control cells were sorted using CD31 (PECAM-1) MicroBeads (Cat # 130-091-935, Miltenyi-Biotec). .. Briefly, “MACS buffer” was prepared with the addition of 5% MACS BSA Stock Solution (Cat # 130-091-376, Miltenyi-Biotec) into autoMACS ® Rinsing Solution (Cat # 130-091-222, Miltenyi-Biotec), then passed through a 0.2 μm filter to prevent clogging of the MS Columns (Cat # 130-042-201, Miltenyi-Biotec). ..

    Article Title: Early cell-autonomous and niche-mediated epithelial response to influenza infection in primary alveolar organoids.
    Article Snippet: .. Flow cytometry of mouse lungs: Single-cell suspensions were obtained as above, and cells were resuspended in 5 mL MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376]) and passed through a 40 μm filter (Greiner Bio-One, 542040). .. Cells were centrifuged and the cell pellet was resuspended in Fc Receptor Binding Inhibitor Polyclonal Antibody (Invitrogen, 14-9161-73) diluted 1:100 in MACS buffer and incubated for 10 min at room temperature.

    Article Title: Elastogenesis by adventitial progenitors acquiring a smooth muscle cell phenotype following aortic dissection
    Article Snippet: .. Sca-1 labeling was conducted directly in the cell suspension using Anti-Sca-1 (non-HSC) MicroBeads (10 μL, #130-106-641, Miltenyi Biotec), and MACS® buffer (1 mL, #130-091-376 and #130-091-221, Miltenyi Biotec) was then added to proceed with magnetic sorting. .. Labeled cells were first filtered through cell strainers (#10199-658, VWR), then separated using MS columns (#130-042-201, Miltenyi Biotec) mounted on a MiniMACSTM Separator (#130-042-102, Miltenyi Biotec) with a MACS® MultiStand (#130-042-303, Miltenyi Biotec).

    Article Title: Coordinated immune activation following KRAS inhibition in syngeneic models reveals molecular pathways that potentiate and limit antitumor immunity
    Article Snippet: Cells were labeled with blue-live dead staining (Invitrogen Cat# L34961) and blocked with mouse Fc-block (BD Biosciences Cat# 553142, RRID:AB_394657) to prevent non-specific binding in 4C for 30 min. .. Cells were washed with MACS buffer (autoMACS Rinsing Solution (130-091-222) + MACS BSA stock solution (Miltenyi Biotec Cat# 130-091-376)) and spun down at 2000rpm for 2min and moved on to surface protein antibody staining at 4°C for 30 min. ..

    Article Title: Targeting DNGR-1 with Fangchinoline Elevates Dendritic Cell Antigen Cross-Presentation-Mediated Antitumor Immunity in Melanoma.
    Article Snippet: .. CD8+ T cells were enriched using CD8 α (Ly-2) icroBeads (130-117-044, MACS) and MACS buffer (130-091-222- , Miltenyi Biotec) followed by magnetic column separation. urified CD8+ T cells were cultured in complete medium suplemented with IL-2 (212-12-100, PeproTech) for activation and ubsequent use. ..

    Article Title: Clinical MAPPs: a personalized healthcare-driven assay for the direct identification of potential T cell epitopes in patients
    Article Snippet: .. Cells were centrifuged twice (300 x g for 5 min at RT) and were re-suspended in 10–50 mL MACS buffer (Miltenyi; Cat: #130-091-221) final volume. .. Monocytes were isolated from PBMCs by positive immune-selection using anti-CD14 microbeads (Miltenyi; Cat: #130-050-201) on LS columns (Miltenyi; Cat: ## 130-042-401) and a QuadroMACS magnetic separator (Miltenyi; Cat: #130-090-976) following the manufacturer’s procedures under sterile conditions.

    Article Title: Neuroinflammatory suppression of astrocytic BMAL1 defines a selective reactive astrocyte program.
    Article Snippet: Then 10 μL of Anti-ACSA-2 Microbeads (Miltenyi) were added, followed by incubation at 4°C for 15 min with rotation. .. Cells were washed, resuspended in 500 μL of MACS buffer, and applied to a pre-wetted MS column (#130-042-201, Miltenyi). ..

    Incubation:

    Article Title: NOD1/2 signaling in macrophages drives adaptive immune resistance in cancer.
    Article Snippet: Single-cell suspensions were generated, and CD45+ immune cells were enriched via magnetic bead-based positive selection using a mouse CD45 TIL MicroBead Kit (130-110-618; Miltenyi Biotec, Bergisch Gladbach, Germany) and a Dead Cell Removal Kit (130-090- 101; Miltenyi Biotec) according to the manufacturer’s instructions. .. Briefly, up to 107 cells were resuspended in 90 μL of MACS Buffer (Miltenyi Biotec) per sample, incubated with 10 μL of microbeads for 15min at 4 °C, and passed through LS columns (130–042–401) mounted on QuadroMACS Separators (Miltenyi Biotec). ..

    Flow Cytometry:

    Article Title: Early cell-autonomous and niche-mediated epithelial response to influenza infection in primary alveolar organoids.
    Article Snippet: .. Flow cytometry of mouse lungs: Single-cell suspensions were obtained as above, and cells were resuspended in 5 mL MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376]) and passed through a 40 μm filter (Greiner Bio-One, 542040). .. Cells were centrifuged and the cell pellet was resuspended in Fc Receptor Binding Inhibitor Polyclonal Antibody (Invitrogen, 14-9161-73) diluted 1:100 in MACS buffer and incubated for 10 min at room temperature.

    Single Cell:

    Article Title: Early cell-autonomous and niche-mediated epithelial response to influenza infection in primary alveolar organoids.
    Article Snippet: .. Flow cytometry of mouse lungs: Single-cell suspensions were obtained as above, and cells were resuspended in 5 mL MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376]) and passed through a 40 μm filter (Greiner Bio-One, 542040). .. Cells were centrifuged and the cell pellet was resuspended in Fc Receptor Binding Inhibitor Polyclonal Antibody (Invitrogen, 14-9161-73) diluted 1:100 in MACS buffer and incubated for 10 min at room temperature.

    Labeling:

    Article Title: Elastogenesis by adventitial progenitors acquiring a smooth muscle cell phenotype following aortic dissection
    Article Snippet: .. Sca-1 labeling was conducted directly in the cell suspension using Anti-Sca-1 (non-HSC) MicroBeads (10 μL, #130-106-641, Miltenyi Biotec), and MACS® buffer (1 mL, #130-091-376 and #130-091-221, Miltenyi Biotec) was then added to proceed with magnetic sorting. .. Labeled cells were first filtered through cell strainers (#10199-658, VWR), then separated using MS columns (#130-042-201, Miltenyi Biotec) mounted on a MiniMACSTM Separator (#130-042-102, Miltenyi Biotec) with a MACS® MultiStand (#130-042-303, Miltenyi Biotec).

    Suspension:

    Article Title: Elastogenesis by adventitial progenitors acquiring a smooth muscle cell phenotype following aortic dissection
    Article Snippet: .. Sca-1 labeling was conducted directly in the cell suspension using Anti-Sca-1 (non-HSC) MicroBeads (10 μL, #130-106-641, Miltenyi Biotec), and MACS® buffer (1 mL, #130-091-376 and #130-091-221, Miltenyi Biotec) was then added to proceed with magnetic sorting. .. Labeled cells were first filtered through cell strainers (#10199-658, VWR), then separated using MS columns (#130-042-201, Miltenyi Biotec) mounted on a MiniMACSTM Separator (#130-042-102, Miltenyi Biotec) with a MACS® MultiStand (#130-042-303, Miltenyi Biotec).

    Staining:

    Article Title: Coordinated immune activation following KRAS inhibition in syngeneic models reveals molecular pathways that potentiate and limit antitumor immunity
    Article Snippet: Cells were labeled with blue-live dead staining (Invitrogen Cat# L34961) and blocked with mouse Fc-block (BD Biosciences Cat# 553142, RRID:AB_394657) to prevent non-specific binding in 4C for 30 min. .. Cells were washed with MACS buffer (autoMACS Rinsing Solution (130-091-222) + MACS BSA stock solution (Miltenyi Biotec Cat# 130-091-376)) and spun down at 2000rpm for 2min and moved on to surface protein antibody staining at 4°C for 30 min. ..

    Cell Culture:

    Article Title: Targeting DNGR-1 with Fangchinoline Elevates Dendritic Cell Antigen Cross-Presentation-Mediated Antitumor Immunity in Melanoma.
    Article Snippet: .. CD8+ T cells were enriched using CD8 α (Ly-2) icroBeads (130-117-044, MACS) and MACS buffer (130-091-222- , Miltenyi Biotec) followed by magnetic column separation. urified CD8+ T cells were cultured in complete medium suplemented with IL-2 (212-12-100, PeproTech) for activation and ubsequent use. ..

    Activation Assay:

    Article Title: Targeting DNGR-1 with Fangchinoline Elevates Dendritic Cell Antigen Cross-Presentation-Mediated Antitumor Immunity in Melanoma.
    Article Snippet: .. CD8+ T cells were enriched using CD8 α (Ly-2) icroBeads (130-117-044, MACS) and MACS buffer (130-091-222- , Miltenyi Biotec) followed by magnetic column separation. urified CD8+ T cells were cultured in complete medium suplemented with IL-2 (212-12-100, PeproTech) for activation and ubsequent use. ..



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